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Aston Villa midfielder Jack Grealish has been called up to the England senior squad for the first time.
However, Marcus Rashford and Harry Winks have pulled out of Gareth Southgate's squad for the Nations League fixtures in Iceland and Denmark.
Grealish, 24, has previously represented England at Under-21 level and was a member of Southgate's 2016 Toulon Tournament-winning squad.
It follows call-ups for Conor Coady and Ainsley Maitland-Niles on Saturday.
Following his withdrawal, Manchester United forward Rashford tweeted: "Gutted. Never want to let this team down but these fixtures just came a little too soon for me.
"I tried my best but I have to focus on starting the season at my strongest for club and country. Good luck boys, I'll be cheering you on from home."
England's players met up at St George's Park on Monday.
Southgate's side face their Euro 2016 conquerors Iceland in Reykjavik on 5 September before playing Denmark in Copenhagen three days later.
The disruption caused to the football calendar by coronavirus, including Euro 2020 being delayed by a year until 2021, means England have not played since November 2019, when a 4-0 win in Kosovo concluded their Euro 2020 qualification campaign.
England squad
Goalkeepers: Dean Henderson, Jordan Pickford, Nick Pope
Defenders: Trent Alexander-Arnold, Conor Coady, Eric Dier, Joe Gomez, Michael Keane, Ainsley Maitland-Niles, Tyrone Mings, Kieran Trippier, Kyle Walker
Midfielders: Phil Foden, Mason Mount, Kalvin Phillips, Declan Rice, James Ward-Prowse, Jack Grealish
Forwards: Tammy Abraham, Mason Greenwood, Danny Ings, Harry Kane, Jadon Sancho, Raheem Sterling
